Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Quantitative Proteomics, Infection, Expressing

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Multiplex Assay, Immunofluorescence

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation

doi: 10.1155/2020/2946820

Figure Lengend Snippet: Depolarization of IL-4-pretreated Raw 264.7 macrophages at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a, b) transcriptional level changes of macrophage M1 polarization-related genes, (a) including iNOS, IL6, and TNF- α , and (b) M2 polarization-related genes, including ARG, IL10, TGF- β , CCL17, EGF, and MMP9. (c) Protein level changes of iNOS. All tests were repeated three times. ( ∗ p < 0.05; † p < 0.01). (d) Flow cytometry analysis of iNOS, CD86, and CD163 proteins.

Article Snippet: LPS (L4391, Sigma) was used with a concentration of 10 ng/mL, SNAP (S-Nitroso-N-Acetyl-D,L-Penicillamine; Sigmal) was used with a concentration of 50 μ M, and cPTIO (2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide, monopotassium salt; Cayman) was used with a concentration of 50 μ M. For staining with antibodies, anti-iNOS-PE (12-5920-80, eBioscience), anti-CD163-PE (bs-2527R-PE, Bioss), and anti-CD86-FITC (bs-1035R-FITC, Bioss) were used.

Techniques: Flow Cytometry

Journal: Bioactive Materials

Article Title: Lactobacillus extracellular vesicle-driven oxygen-releasing photothermal hydrogel reprograms macrophages and promotes angiogenesis to accelerate diabetic wound healing

doi: 10.1016/j.bioactmat.2025.08.010

Figure Lengend Snippet: Immunomodulatory properties of hydrogels. Immunofluorescent staining Images and quantitative analysis of pro-inflammatory cytokine marker protein CD86 (a–b) and anti-inflammatory cytokine marker protein CD206 (c–d) (n = 3). Scale bar, 100 μm. (e) ELISA was used to detect the expression of pro-inflammatory cytokine marker TNF-α after treatment with different group (n = 3). (f–g) The mRNA expression levels of pro-inflammatory cytokine markers (IL-1β and IL-6) were detected by qRT-PCR (n = 3). (h) ELISA was used to detect the expression of anti-inflammatory cytokine marker IL-10 after treatment with different group (n = 3). (i–j) The mRNA expression levels of anti-inflammatory cytokine markers (Arg-1 and IL-10) were detected by qRT-PCR (n = 3). Data are presented as mean ± SEM. Statistical analysis: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. I: PBS, II: PCP, III: PCPH, IV: PCPH@ Lac -EVs, V: PCPH@ Lac -EVs with 808 nm NIR irradiation.

Article Snippet: CD86 Polyclonal antibody and CD206 Polyclonal antibody were purchased from Wuhan Sanying Biotechnology Co., Ltd. RNA EASY FAST Total RNA Extraction Kit was obtained from Tiangen Biotech (Beijing) Co., Ltd. Prime Script TMRT Master Mix and TB Green® premix ExTaq TMII were purchased from Takara Bio Inc. Hoechst 33342 was purchased from Shanghai Beyotime Biotechnology Co., Ltd. PKH26 was purchased from Umibio (Shanghai) Co., Ltd. QuantiCyto® Mouse TNF-α ELISA kit and QuantiCyto® Mouse IL-10 ELISA kit were purchased from Neobioscience Technology Co., Ltd. All reagents were analytically pure and used directly.

Techniques: Staining, Marker, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Irradiation

Journal: Bioactive Materials

Article Title: Lactobacillus extracellular vesicle-driven oxygen-releasing photothermal hydrogel reprograms macrophages and promotes angiogenesis to accelerate diabetic wound healing

doi: 10.1016/j.bioactmat.2025.08.010

Figure Lengend Snippet: Immunological analysis of the wound tissues. (a) Immunofluorescence staining of CD86 and CD206 in different groups at day 7, DAPI: blue, CD86: green, CD206: red. Scale bar, 25 μm. (b–c) Relative quantitative analysis of CD86 (b) and CD206 (c) at day 7 (n = 3). (d) Representative image of TNF-α and TGF-β immunostaining in wound tissue from different groups at day 7, DAPI: blue, TNF-α: green, TGF-β: red. Scale bar, 25 μm. (e–f) Relative quantitative analysis of TNF-α (e) and TGF-β (f) at day 7 (n = 3). Data are presented as mean ± SEM. Statistical analysis: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. I: PBS, II: PCP, III: PCPH, IV: PCPH@ Lac -EVs, V: PCPH@ Lac -EVs with 808 nm NIR irradiation.

Article Snippet: CD86 Polyclonal antibody and CD206 Polyclonal antibody were purchased from Wuhan Sanying Biotechnology Co., Ltd. RNA EASY FAST Total RNA Extraction Kit was obtained from Tiangen Biotech (Beijing) Co., Ltd. Prime Script TMRT Master Mix and TB Green® premix ExTaq TMII were purchased from Takara Bio Inc. Hoechst 33342 was purchased from Shanghai Beyotime Biotechnology Co., Ltd. PKH26 was purchased from Umibio (Shanghai) Co., Ltd. QuantiCyto® Mouse TNF-α ELISA kit and QuantiCyto® Mouse IL-10 ELISA kit were purchased from Neobioscience Technology Co., Ltd. All reagents were analytically pure and used directly.

Techniques: Immunofluorescence, Staining, Immunostaining, Irradiation